Journal: Frontiers in Chemistry

Article Title: Identification of 6ω-cyclohexyl-2-(phenylamino carbonylmethylthio)pyrimidin-4(3 H )-ones targeting the ZIKV NS5 RNA dependent RNA polymerase

doi: 10.3389/fchem.2022.1010547

Figure Lengend Snippet: The interaction between 4w and ZIKV NS5 protein. (A) WB detection of ZIKV NS5 protein expression under different temperature gradients after 4w treatment; (B) NS5 protein grayscale analysis and ZIKV NS5 protein expression after 4w treatment observed changes in aggregation temperature; data is the mean (±SD) of three experiments. * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001.

Article Snippet: Subsequently, the protein was transferred to a polyvinylidene fluoride (PVDF) membrane and incubated with ZIKV E (1:2,000) and NS5 antibody (1:2,000) overnight at 4°C (Sino Biological, China) then add the corresponding secondary antibody and incubate at room temperature for 2 h. A specific signal was presented with a chemiluminescent substrate.

Techniques: Expressing

Journal: Frontiers in Chemistry

Article Title: Identification of 6ω-cyclohexyl-2-(phenylamino carbonylmethylthio)pyrimidin-4(3 H )-ones targeting the ZIKV NS5 RNA dependent RNA polymerase

doi: 10.3389/fchem.2022.1010547

Figure Lengend Snippet: Compound 4w inhibited the expression of ZIKV E and NS5 protein. (A) Western blot detected the inhibitory effect of 4w on ZIKV E and NS5 protein under the concentration gradient of 4w ; (B) Grayscale analysis and statistics of the inhibition of 4w on ZIKV E and NS5 protein under the concentration gradient of WB detection; Data is the mean (±SD) of three experiments, with DMSO as a positive contro * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001. (C) Detected the inhibitory effect of 4w on ZIKV E protein by immunofluorescence.

Article Snippet: Subsequently, the protein was transferred to a polyvinylidene fluoride (PVDF) membrane and incubated with ZIKV E (1:2,000) and NS5 antibody (1:2,000) overnight at 4°C (Sino Biological, China) then add the corresponding secondary antibody and incubate at room temperature for 2 h. A specific signal was presented with a chemiluminescent substrate.

Techniques: Expressing, Western Blot, Concentration Assay, Inhibition, Immunofluorescence

Journal: Applied Microbiology and Biotechnology

Article Title: Development and characterization of mouse monoclonal antibodies targeting to distinct epitopes of Zika virus envelope protein for specific detection of Zika virus

doi: 10.1007/s00253-021-11364-1

Figure Lengend Snippet: Alignment of the binding epitopes of mAbs A1 ( a ), B1 ( b ), C1 ( c ), and 9E-1 ( d ) with other flavivirus E proteins. DENV, dengue virus; WNV, West Nile virus; YFV, yellow fever virus; JEV, Japanese encephalitis virus. The NCBI accession numbers of the E proteins derived for the indicated virus strains are provided in the parentheses. e The recombinant E proteins of ZIKV (2 μg), DENV2 (1.75 μg), DENV3 (0.42 μg), and DENV4 (0.78 μg) were separated on SDS-PAGE and then transferred to a PVDF membrane for performing the WB analysis with mAbs Anti-His, A1, B1, C1, or 9E-1, respectively. The numbers labeled on the left of the designated ticks are the molecular weight standards in kDa unit

Article Snippet: The recombinant DENV serotype 2 (DENV2) E protein (GenBank: AAC59274.1), serotype 3 (DENV3) E protein (GenBank: AAA99437.1), and serotype 4 (DENV4) E protein (GenBank: AAX48017.1) were purchased from Sino Biological Inc. (Catalog Number: 40471-V08B, 40532-V08H1, and 40533-V08B2, respectively).

Techniques: Binding Assay, Virus, Derivative Assay, Recombinant, SDS Page, Membrane, Labeling, Molecular Weight

Journal: Emerging Microbes & Infections

Article Title: Convalescent patient-derived monoclonal antibodies targeting different epitopes of E protein confer protection against Zika virus in a neonatal mouse model

doi: 10.1080/22221751.2019.1614885

Figure Lengend Snippet: Binding and neutralizing activities of E-targeted mAbs.

Article Snippet: DENV1 EDIII protein (Cat. no. 40531-V08B), DENV2 EDIII protein (Cat. no. 40471-V08Y3), and DENV4 E protein (Cat. no. 40533-V08B2) were also purchased from Sino Biological Inc.

Techniques: Binding Assay, Neutralization